Sulfate salt of quinolonecarboxylic acid derivatives and the use thereof

ABSTRACT

To provide novel sulfate or its hydrate useful for efficiently recycling 1-cyclopropyl-7-(3-methyl-1- piperazinyl)-6-fluoro-8-methoxy-1,4-dihydro-4-oxo-3- quinolinecarboxylic acid, the sulfate of 1-cyclopropyl-7- (3-methyl-1-piperazinyl)-6-fluoro-8-methoxy-1,4-dihydro- 4-oxo-3-quinolinecarboxylic acid or its hydrate is provided and yet its industrially useful application is provided.

TECHNICAL FIELD

[0001] The present invention relates to sulfate of 1-cyclopropyl- 7-(3-methyl-1-piperazinyl)-6-fluoro-8-methoxy-1,4- dihydro-4-oxo-3-quinolinecarboxylic acid (International Nonproprietary Name: gatifloxacin), its use for recovering gatifloxacin and recovering method. Gatifloxacin is characterized by its chemical structure having an alkoxy group at 8-position, and is useful as an antimicrobial agent.

BACKGROUND TECHNOLOGIES

[0002] As a method of treating industrial waste liquor containing quinolonecarboxylic acid derivatives, it is common to recover by adsorbing effective ingredients onto synthetic adsorbent etc., or to dispose by directly incinerating waste liquor itself without performing such recovering procedure of the like.

[0003] The method of recovering effective ingredients from industrial waste liquor containing quinolonecarboxylic acid derivatives by using synthetic adsorbent etc. is complicated procedurally and not a good method, resulting also in a cause of steep rise in preparation cost. Also, the incinerating disposal of industrial waste liquor containing a large quantity of effective ingredients cannot be said to be a preferable method from the environmental preservation.

[0004] Hence, upon preparation of gatifloxacin, it is very useful in the industry to find out a method of advantageously recovering gatifloxacin industrially from waste liquor after preparation and regenerating it.

DISCLOSURE OF THE INVENTION

[0005] As a result of diligent investigation on a method of industrially recovering gatifloxacin useful as an antimicrobial agent under such situation, the inventors have found that, if forming sulfate of gatifloxacin in the waste liquor etc., allowing to sediment and separate, and then freeing from salt, gatifloxacin can be advantageously recovered industrially, leading to the completion of the invention.

[0006] The inventive gatifloxacin sulfate and its hydrate are novel compounds not described in the literature. The concrete preparing method lies in that aqueous sulfuric acid is added to acidity water or water-containing alcohol dissolved or suspended gatifloxacin, thereby sedimenting sulfate of gatifloxacin. If recrystallizing this further from, for example, water, hydrate of sulfate of gatifloxacin can be obtained.

[0007] Also, when recovering gatifloxacin from industrial waste liquor after preparation, aqueous sulfuric acid is added to acidity water-containing alcoholic solution dissolved gatifloxacin, thereby obtaining sulfate as sedimented crystals. These are collected by filtration and washed or recrystallized, and then dissolved or suspended into water. Following this, if the solution is made weak alkaline with aqueous solution of alkali such as sodium hydroxide, good-quality gatifloxacin is freed, thus enabling to recover. In addition, the recycling use is possible by adding sulfate to new preparing process without separating gatifloxacin, leaving as it is in alkaline solution.

BEST EMBODIMENT TO PUT THE INVENTION INTO PRACTICE

[0008] Moreover, the alcoholic organic solvent after recovery of gatifloxacin as sulfate can be recovered as a solvent containing no basic ingredients, hence efficient recycling use is possible.

EXAMPLE

[0009] In following, the invention will be illustrated in detail by describing examples and referential example, but it is not confined thereto.

Example 1 Manufacture of Preparative Sample of Sulfate

[0010] Into 200 mL of water were suspended 20 g of 1-cyclopropyl-piperazinyl)-6-fluoro-8-methoxy-1,4-dihydro- 4-oxo-3-quiolinecarboxylic acid, which was dissolved by adding 68% acetic acid (5 mL). To this solution, 35% sulfuric acid (10 mL) was added to make acidic. Then, the sedimented crystals were collected by filtration and washed with water.

[0011] The crystals obtained were recrystallized from 400 mL of water to obtain 18.4 g of ½·sulfate ⅖ hydrate of 1-cyclopropyl-7-(3-methyl-1-piperazinyl)-6-fluoro-8-methoxy-1,4-dihydro-4-oxo-3-quinolinecarboxylic acid.

[0012] Melting point: 212-215° C. (decomp).

[0013] Element analysis: C% H% N% Calcd. For C₁₉H₂₂FN₃O₄ · ½H₂SO₄ · ⅖H₂O: 52.87 5.46 9.74. Found: 53.00 5.57 9.99.

[0014] MS (m/e):

[0015] 375 [C₁₉H₂₂FN₃O₄]⁺

[0016] 344 [C₁₉H₂₂FN₃O₄—OCH₃]⁺

[0017] 331 [C₁₉H₂₂FN₃O₄—CO₂]⁺

[0018] 319 [C₁₉H₂₂FN₃O₄—C₃H₆N]⁺

[0019] 275 [C₁₉H₂₂FN₃O₄—CO₂—C₃H₆N]⁺

[0020] 260 [C₁₉H₂₂FN₃O₄—CO₂—C₄H₉N]⁺

[0021] NMR (¹H-NMR: 400 MHz, DMSO-d₆): δ

[0022] 1.01-1.15 (7H, m, (—CH₂)₂ of cyclopropyl group,

[0023] CH₃— of piperazine ring)

[0024] 3.02-3.45 (7H, m, piperazine ring)

[0025] 3.78 (3H, s, CH₃O—)

[0026] 4.14-4.20 (1H, m, —CH═ of cyclopropyl group)

[0027] 7.75 (1H, d, J=11.7 Hz, 5-position CH of quinolone ring)

[0028] 8.71 (1H, s, 2-position CH of quinolone ring)

Example 2

[0029] An ethanol filtrate containing 1-cyclopropyl-7-(3-methyl-1-piperazinyl)-6-fluoro-8-methoxy-1,4-dihydro-4-oxo-3-quinolinecarboxylic acid caused by recrystallization in Referential example 1 was warmed to 40° C., and 35% sulfuric acid was added dropwise to bring to pH 4, which was stirred for 2 hours at the same temperature.

[0030] The sedimented crystals were collected by filtration to obtain 22.8 g of sulfate of 1-cyclopropyl-7-(3-methyl-1- piperazinyl)-6-fluoro-8-methoxy-1,4-dihydro-4-oxo-3- quinolinecarboxylic acid.

[0031] Into 105 mL of water, 21 g of this sulfate were suspended and dissolved by adding 24.5% aqueous solution of sodium hydroxide.

[0032] This solution was recycled to hydrolyzing solution of next lot as it is.

Example 3

[0033] After 3.6L of ethanol filtrate containing 29 g (content was calculated from recrystallization yield) of 1-cyclopropyl- 7-(3-methyl-1-piperazinyl)-6-fluoro-8-methoxy-1,4-dihydro- 4-oxo-3-quinolinecarboxylic acid obtained by the procedure similar to Referential example 1 was warmed to 40° C., 35% sulfuric acid was added dropwise to bring to pH 4, which was stirred for 2 hours at 40 to 45° C.

[0034] The sedimented crystals were collected by filtration to obtain 27.9 g of sulfate of 1-cyclopropyl-7-(3-methyl-1- piperazinyl)-6-fluoro-8-methoxy-1,4-dihydro-4-oxo-3- quinolinecarboxylic acid.

[0035] Into 133 mL of water, 26.5 g of this sulfate were suspended and dissolved by adding 24.5% aqueous solution of sodium hydroxide. To the dissolved solution, 68% acetic acid was added to adjust to pH 8, which was stirred for 1 hour as it is and then stirred for 10 minutes of 80 to 84° C. The sedimented crystals were collected by filtration and then washed with water to obtain 6.5g of 1-cyclopropyl-7-(3-methyl-1- piperazinyl)-6-fluoro-8-methoxy-1,4-dihydro-4-oxo-3- quinolinecarboxylic acid.

Example 4

[0036] After 1000L of filtrate containing 3 kg (content was calculated from recrystallization yield) of 1-cyclopropyl- 7-(3-methyl-1-piperazinyl)-6-fluoro-8-methoxy -1,4-dihydro-4-oxo-3-quinolinecarboxylic acid recrystallized using 2-propanol in place of ethanol was warmed to 40° C., 35% sulfuric acid was added dropwise to bring to pH 4, which was stirred for 2 hours at the same temperature.

[0037] The sedimented crystals were collected by filtration and then washed with 45L of water in suspended state, thereby obtaining 3.03 kg of sulfate of 1-cyclopropyl-7-(3-methyl- 1-piperazinyl)-6-fluoro-8-methoxy-1,4-dihydro-4-oxo-3-quinolinecarboxylic acid.

Referential Example 1

[0038] To 215 g of (1-cyclopropyl-6,7-fluoro-1,4-dihydro-8- methoxy-4-oxo-3-quinolinecarboxylate-O³,O⁴)bis(acetate-O)-borate were added 66.1 g of 2-methylpiperazine, 113 g of triethylamine and 403 g of acetonitrile, and the mixture was stirred overnight at room temperature.

[0039] The solvent was distilled off and, after added 1.24L of 0.5% acetic acid, the mixture was stirred for 3 hours at 80 to 85° C. The reaction mixture was cooled and, after added 1 g of activated carbon, the mixture was stirred for 0.5 hours. The activated carbon was separated by filtration, and to the filtrate, 24.5% aqueous solution of sodium hydroxide was added to adjust to pH 8, which was then stirred overnight at 0 to 5° C.

[0040] The sedimented crystals were collected by filtration to obtain 191g of crude crystals of 1-cyclopropyl-7-(3- methyl-1-piperazinyl)-6-fluoro-8-methoxy-1,4-dihydro-4- oxo-3-quinolinecarboxylic acid.

[0041] These crude crystals were recrystallized from 90% ethanol to obtain 160g of 1-cyclopropyl-7-(3-methyl-1- piperazinyl)-6-fluoro-8-methoxy-1,4-dihydro-4-oxo-3- quinolinecarboxylic acid.

[0042] Utilizability in the Industry

[0043] The inventive sulfate of 1-cyclopropyl-7-(3-methyl-1- piperazinyl)-6-fluoro-8-methoxy-1,4-dihydro-4-oxo-3- quinolinecarboxylic acid (gatifloxacin) and its hydrate are novel acid adducts. It has become clear that, if forming sulfate of gatifloxacin from the waste liquor after preparation, separating and purifying by usual method, and then freeing, gatifloxacin can be advantageously recovered industrially.

[0044] The gatifloxacin recovered via sulfate can be brought to recycling use as a product. Moreover, the alcoholic organic solvent after recovery of sulfate can be recovered as a solvent containing no basic ingredients, hence efficient recycling application is possible. 

Scope of the claim
 1. Sulfate of 1-cyclopropyl-7-(3-methyl-1- piperazinyl)-6-fluoro-8-methoxy-1,4-dihydro-4-oxo-3- quinolinecarboxylic acid (sulfate of gatifloxacin) or hydrate of that sulfate.
 2. Use of sulfate of gatifloxacin or hydrate of that sulfate on recovering 1-cyclopropyl-7-(3-methyl-1- piperazinyl)-6-fluoro-8-methoxy-1,4-dihydro-4-oxo-3- quinolinecarboxylic acid (gatifloxacin).
 3. Method of recovering gatifloxacin, characterized in that aqueous sulfuric acid is added to acidity water or water-containing alcohol dissolved or suspended 1-cyclopropyl-7-(3-methyl-1-piperazinyl)-6-fluoro-8- methoxy-1,4-dihydro-4-oxo-3-quinolinecarboxylic acid (gatifloxacin), thereby sedimenting sulfate of gatifloxacin or hydrate of that sulfate. 